zeta-Cypermethrin [733]

Content Handbook P

 

Outline of method

The sample is dissolved in a prepared solution of a mixture of iso-octane, n-butyl chloride, toluene, and acetonitrile in a ratio by volume of 800:200:20:1, respectively. The mobile phase is a solution of 1,2-dichloroethane and hexanes in a ratio also by volume of 110:1000. The analysis is conducted via normal phase chiral chromatography using a 5 μm particle size 25 cm by 4.6 mm i.d. stainless steel column packed with Chiral D-PGC (phenyl glycine column) available from a variety of suppliers. UV detection at 280 nm is used to determine the peak areas
of S-enantiomer and R-enantiomer of zeta-cypermethrin diastereoisomer (peak D in CIPAC Method No. 332) and are measured by a data handling system. These areas are then used to further calculate the area percent ratio between the two Trans-2 (Peak D) enantiomers in zeta cypermethrin technical. Subsequently, the area percent ratio is used to calculate the weight percent of the R or S enantiomers in zeta-cypermethrin technical i.e., the result of the CIPAC Method No. 332 is multiplied by the area ratio to give the desired weight percent of R and S enantiomers in zeta-cypermethrin.

 

Scope

The method is usable for TC

ALPHA-CYPERMETHRIN [454] and CHLORFENAPYR [570] (incorporated LN)

Content Handbook P

 

Outline of method

The pyriproxyfen in LN is determined by reverse phase high performance liquid chromatography using UV detector, at detection wavelength of 254 nm with dicyclohexyl phthalate as internal standard.
The same sample extracted in heptane is used for the determination of alphacypermethrin by GC-FID

 

Scope

The method 454/TC/(M)/3 (CIPAC H, p 15) was extended to determine the alphacypermethrin with minor modifications.


The method 715/LN/M/3 (CIPAC O, p 143) was extended to determine the pyriproxyfen without any modifications.

Beta-cyfluthrin

 

Content Handbook H

 

Outline of method

Beta-cyfluthrin is determined by normal phase high performance liquid chromatography using UV detection at 235 nm and external standardisation.

 

 

Scope

The method is intended for the determination of beta-cyfluthrin and its diastereoisomer ratio.

The method is usable for TC, SC and EC-formulations.

 

 

ALPHA-CYPERMETHRIN [454] and CHLORFENAPYR [570]

Content Handbook P

 

Outline of method

The sample is extracted with heptane using dicyclohexyl phthalate as internal standard. Alpha-cypermethrin and/or chlorfenapyr contents are determined by capillary gas chromatography using flame ionization detection (GC-FID).

 

Scope

This method is intended for determining a-cypermethrin and/or chlorfenapyr content in long-lasting insecticidal nets (LNs).